The aim of the paper is to diagnose the organizational culture profile in a healthcare institution based on employees’ opinions and to identify discrepancies between the current and the preferred culture. The study was conducted using a quantitative method with the Organizational Culture Assessment Instrument (OCAI) based on Cameron and Quinn’s Competing Values Framework. The survey included the full population of 233 hospital employees, representing both clinical and non-clinical staff. The results indicate the dominance of a hierarchical culture and significant expectations regarding the strengthening of clan and adhocracy culture characteristics. The findings are descriptive and diagnostic in nature and allow for the identification of potential managerial challenges related to aligning organizational culture with employees’ expectations and the contemporary conditions of healthcare organizations.