The article addresses the issue of economic and financial sanctions adopted by the EU in response to the conflict in Ukraine from the perspective of challenges in the implementation of sanctions. The form, specificity and scope of the sanctions adopted are examined, as well as the measures and tools taken by EU institutions to ensure the proper implementation of economic and financial sanctions. The manner in which subsequent sanction packages are adopted require specific actions to enable uniform application of sanctions in individual Member States and their effectiveness. The aim of the article is to demonstrate that in order to ensure uniformity and effectiveness of the adopted measures, it is necessary for EU institutions to take actions such as, creation soft law documents and deliver a technical support. These actions also constitute a fundamental mechanism for supporting entrepreneurs in the EU affected by the consequences of the adopted restrictive measures. The article provides an overview of the supporting tools used by the EU institutions, while analysing the legal basis for the actions taken by individual institutions. Taking adequate actions by the EU institutions determines the way in which individual companies interpret and implement sanctions resulting directly from EU law.